The phytochemical examination and antioxidant activity of isolated phytoconstituents from the ethanolic extract of whole plant of Hyptis suaveolens. The air dried and powdered plants materials were separately extracted successively with various solvents viz. petroleum ether (400–600 C), chloroform, ethanol and water with increasing order of polarity using a soxhlet extractor. The ethanolic extract of Hyptis suaveolens upon concentration under reduced pressure left a dark green sticky and oily residue (18.7%). It gave positive Liebermann-Burchard reaction (Pink-Blue-Green). In TLC examination of the residue showed number of spots (Toluene:Ethyl acetate:Metenol: Water (7:6:5:1)) on spraying with 5% alcoholic H2SO4 followed by heating. The ethanolic residue was subjected to column chromatography over silica gel (60-200 mesh). The residue was subjected to column chromatography over silica gel afforded three compounds named C-01, C-02 and C-03. The compounds were characterized through chemical and spectral analysis and confirmed as b-Sitosterol (C-01) Lupeol (C-02) and Betulinic acid (C-03) respectively. The antioxidant activities of the all three compounds were evaluated by using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical elimination and thiocyanate method. All three compounds showed significant antioxidant activity in the DPPH and H2O2 Radical scavenging method. From the whole plant ethanolic extract all these compounds were tested for antioxidant activity first time from this plant.
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